Application
A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-p27 Kip1 (pS10) RabMAb (Cat. #AJ1569d), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP.Application
C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-p27Kip1 RabMAb. (Cat. #AJ1569d).Application
B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Phospho-p27Kip1 RabMAb (Cat. #AJ1569d)| Product Name | p27/Kip1 Antibody Phospho (pS10) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27;p27Kip1 |
| Modification | p-S10 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP233(2)Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIP1 |
|---|---|
| Alternative Names | CDKN1B KIP1 Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 |
| Molecular Weight(MW) | 22073 Da |
| Function | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry |
| Tissue Specificity | Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney |
| Cellular Localization | Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) |
| Entrez Gene | 1027 |
|---|
Application
A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-p27 Kip1 (pS10) RabMAb (Cat. #AJ1569d), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP.
Application
C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-p27Kip1 RabMAb. (Cat. #AJ1569d).
Application
B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Phospho-p27Kip1 RabMAb (Cat. #AJ1569d)| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
