Application
Figure 1: Western blot analysis using CDKN1B mAb against CDKN1B-hIgGFc transfected HEK293 cell lysate.| Product Name | CDKN1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIP1; MEN4; CDKN4; MEN1B; P27KIP1; CDKN1B |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3D8 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIP1 |
|---|---|
| Alternative Names | KIP1 MEN4 CDKN4 MEN1B P27KIP1 CDKN1B |
| Molecular Weight(MW) | 22073 Da |
| Function | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry |
| Tissue Specificity | Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney |
| Cellular Localization | Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) |
| Entrez Gene | 1027 |
|---|---|
| Nucleotide Accession | NP_004055.1 |
Application
Figure 1: Western blot analysis using CDKN1B mAb against CDKN1B-hIgGFc transfected HEK293 cell lysate.| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human CDKN1B expressed in E. Coli. |
