Application
Western blot analysis of P3H3 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). P3H3 (arrow) was detected using the purified Pab.Application
P3H3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P3H3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
P3H3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | P3H3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEPREL2; P3H3; Prolyl 3-hydroxylase 3; Leprecan-like protein 2; Protein B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P3H3 |
|---|---|
| Alternative Names | LEPREL2 P3H3 Prolyl 3-hydroxylase 3 Leprecan-like protein 2 Protein B |
| Molecular Weight(MW) | 81837 Da |
| Function | Has prolyl 3-hydroxylase activity catalyzing the post- translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V (By similarity) |
| Tissue Specificity | Weak expression in heart, lung, ovary and skeletal muscle |
| Cellular Localization | Endoplasmic reticulum (By similarity). |
| Entrez Gene | 10536 |
|---|
Application
Western blot analysis of P3H3 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). P3H3 (arrow) was detected using the purified Pab.
Application
P3H3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P3H3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
P3H3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
