Application
Western blot analysis of P450R antibody (N-term) (Cat.# AP6518a) in HepG2 cell line lysates (35ug/lane). P450R (arrow) was detected using the purified Pab.Application
P450R Antibody (N-term) (Cat.# AP6518a) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P450R Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
P450R Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | P450R Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POR; CYPOR; NADPH--cytochrome P450 reductase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYPOR |
|---|---|
| Alternative Names | POR CYPOR NADPH--cytochrome P450 reductase |
| Molecular Weight(MW) | 76690 Da |
| Function | This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5 |
| Tissue Specificity | This P450R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~160 amino acids from the N-terminal region of human P450R. |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Anchored to the ER membrane by its N- terminal hydrophobic region |
| Entrez Gene | 5447 |
|---|
Application
Western blot analysis of P450R antibody (N-term) (Cat.# AP6518a) in HepG2 cell line lysates (35ug/lane). P450R (arrow) was detected using the purified Pab.
Application
P450R Antibody (N-term) (Cat.# AP6518a) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the P450R Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
P450R Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM216287