Application
Western blot analysis of P4HB Antibody (C-term) in CEM,HepG2,A2058 cell line lysates (35ug/lane). P4HB (arrow) was detected using the purified Pab.(2ug/ml)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with P4HB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using P4HB Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | P4HB Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P4HB; ERBA2L; PDI; PDIA1; PO4DB; Protein disulfide-isomerase; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERBA2L PDI PDIA1 PO4DB |
|---|---|
| Alternative Names | P4HB ERBA2L PDI PDIA1 PO4DB Protein disulfide-isomerase Cellular thyroid hormone-binding protein Prolyl 4-hydroxylase subunit beta p55 |
| Molecular Weight(MW) | 57116 Da |
| Function | This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP |
| Tissue Specificity | This P4HB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443~472 amino acids from the C-terminal region of human P4HB. |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein (Potential) Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 5034 |
|---|
Application
Western blot analysis of P4HB Antibody (C-term) in CEM,HepG2,A2058 cell line lysates (35ug/lane). P4HB (arrow) was detected using the purified Pab.(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with P4HB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using P4HB Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
