WB
Western Blot; Sample: Mouse Testis lysate. Primary Ab: 1µg/ml Rabbit Anti-Human PADI1 Antibody. Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.| Product Name | Polyclonal Antibody to Peptidyl Arginine Deiminase Type I (PADI1) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant PADI1 (Pro298~Arg556) expressed in E.coli |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:0.01-2µg/mL |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PADI1 |
|---|---|
| Gene Synonyms | PDI PAD1 PDI1 HPAD10 |
| Gene Full Name | peptidyl arginine deiminase 1 |
| Gene Summary | This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008] |
| Alternative Names | PDI PAD1 HPAD10 PDI1 Peptidylarginine Deiminase Type I Protein-Arginine Deiminase Type-1 hPAD-Colony 10 |
| Molecular Weight(MW) | 70 kDa |
WB
Western Blot; Sample: Mouse Testis lysate. Primary Ab: 1µg/ml Rabbit Anti-Human PADI1 Antibody. Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.| Application Notes | WB:0.01-2µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. |
| Storage Buffer | Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol. |
