Immunoblot
1. 100 ug HeLa cell lysate2. 100 ug mouse liver cytosolic extract 3. 1.5 ug human 26S ProteasomePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit APSecondary Antibody Dilution: 1:10000Gene Name: PSMD14Submitted by: Anonymous| Product Name | Rabbit anti-PSMD14 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAD1, POH1, RPN11 |
| Immunogen | The immunogen for anti-PSMD14 antibody: synthetic peptide directed towards the C terminal of human PSMD14 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMD14 |
|---|---|
| Gene Synonyms | PAD1 POH1 RPN11 |
| Gene Full Name | Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 |
| Gene Summary | POH1 (pad one homolog-1) is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway |
| Alternative Names | PAD1 POH1 RPN11 |
| Molecular Weight(MW) | 35kDa |
| Sequence | 310 amino acids |
Immunoblot
1. 100 ug HeLa cell lysate2. 100 ug mouse liver cytosolic extract 3. 1.5 ug human 26S ProteasomePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit APSecondary Antibody Dilution: 1:10000Gene Name: PSMD14Submitted by: Anonymous| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
