WB
Western Blot: Positive WB detected in: MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), U251 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Jurkat whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse stomach tissue lysate(30µg), Rat stomach tissue lysate(30µg). All lanes:PSMD14 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/40000 dilution. Exposure time：1min.IHC
IHC image of PSMD14 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.ICC/IF
Immunofluorescence staining of PC-3 cell with PSMD14 Recombinant Monoclonal Antibody at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).FC
Overlay Peak curve showing 786-O cells stained with PSMD14 Recombinant Monoclonal Antibody (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.| Product Name | PSMD14 Recombinant Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide from human PSMD14 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:50-1:200 ICC/IF:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMD14 |
|---|---|
| Gene Synonyms | PAD1 POH1 RPN11 |
| Gene Full Name | proteasome 26S subunit, non-ATPase 14 |
| Gene Summary | This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012] |
| Alternative Names | 26S proteasome non-ATPase regulatory subunit 14 antibody 26S proteasome regulatory subunit rpn11 antibody 26S proteasome-associated PAD1 homolog 1 antibody 26S proteasome-associated PAD1 homolog antibody PAD1 antibody PAD1 yeast homolog of antibody POH1 antibody Proteasome (prosome macropain) 26S subunit non-ATPase 14 antibody Proteasome 26S subunit non ATPase 14 antibody PSDE_HUMAN antibody Psmd14 antibody RPN11 antibody Testis tissue sperm binding protein Li 69n antibody |
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Proteasome |
WB
Western Blot: Positive WB detected in: MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), U251 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Jurkat whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Mouse stomach tissue lysate(30µg), Rat stomach tissue lysate(30µg). All lanes:PSMD14 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/40000 dilution. Exposure time：1min.
IHC
IHC image of PSMD14 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
ICC/IF
Immunofluorescence staining of PC-3 cell with PSMD14 Recombinant Monoclonal Antibody at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC
Overlay Peak curve showing 786-O cells stained with PSMD14 Recombinant Monoclonal Antibody (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.| Application Notes | WB:1:500-1:5000 IHC:1:50-1:200 ICC/IF:1:50-1:200 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
