Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in WB on Human HeLa, Mouse liver at 1:1000 (Lane 1: 100 ug HeLa cell lysate, Lane 2: 100 ug mouse liver cytosolic extract 3. 1.5 ug human 26S Proteasome).| Product Name | PSMD14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMD14, PAD1, POH1, RPN11 |
| Product description | POH1 (pad one homolog-1) is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway1) Spataro, V., et al., (1997) J. Biol. Chem. 272 (48), 30470-30475. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSMD14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PSMD14 antibody can be used for detection of PSMD14 by ELISA at 1:312500. PSMD14 antibody can be used for detection of PSMD14 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DrosophilaHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMD14 |
|---|---|
| Alternative Names | PSMD14 PAD1 POH1 RPN11 |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on Human HeLa, Mouse liver at 1:1000 (Lane 1: 100 ug HeLa cell lysate, Lane 2: 100 ug mouse liver cytosolic extract 3. 1.5 ug human 26S Proteasome).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PSMD14 antibody can be used for detection of PSMD14 by ELISA at 1:312500. PSMD14 antibody can be used for detection of PSMD14 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PSMD14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
