WB
Western blot analysis of PSMD14 on different lysates with Rabbit anti-PSMD14 antibody at 1/1,000 dilution.Lysates/proteins at 40 µg/Lane. Exposure time: 40 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody:Rabbit anti-PSMD14 antibody,1/1,000 in primary antibody dilution buffer,overnight at 4 ℃.Secondary antibody: Goat anti-Rabbit IgG-HRP , 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSMD14 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PSMD14 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PSMD14 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | PSMD14 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human PSMD14 aa 92-230 / 310. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PSMD14 |
|---|---|
| Gene Synonyms | PAD1 POH1 RPN11 |
| Gene Full Name | proteasome 26S subunit, non-ATPase 14 |
| Gene Summary | This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012] |
| Alternative Names | 26S proteasome non-ATPase regulatory subunit 14 antibody 26S proteasome regulatory subunit rpn11 antibody 26S proteasome-associated PAD1 homolog 1 antibody 26S proteasome-associated PAD1 homolog antibody PAD1 antibody PAD1 yeast homolog of antibody POH1 antibody Proteasome (prosome macropain) 26S subunit non-ATPase 14 antibody Proteasome 26S subunit non ATPase 14 antibody PSDE_HUMAN antibody 26S proteasome non-ATPase regulatory subunit 14 antibody 26S proteasome regulatory subunit rpn11 antibody 26S proteasome-associated PAD1 homolog 1 antibody 26S proteasome-associated PAD1 homolog antibody PAD1 antibody PAD1 yeast homolog of antibody POH1 antibody Proteasome (prosome macropain) 26S subunit non-ATPase 14 antibody Proteasome 26S subunit non ATPase 14 antibody PSDE_HUMAN antibody Psmd14 antibody RPN11 antibody Testis tissue sperm binding protein Li 69n antibody |
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cytosol. Extracellular region or secreted. Nucleus. |
WB
Western blot analysis of PSMD14 on different lysates with Rabbit anti-PSMD14 antibody at 1/1,000 dilution.Lysates/proteins at 40 µg/Lane. Exposure time: 40 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody:Rabbit anti-PSMD14 antibody,1/1,000 in primary antibody dilution buffer,overnight at 4 ℃.Secondary antibody: Goat anti-Rabbit IgG-HRP , 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSMD14 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PSMD14 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PSMD14 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
