Application
Western blot analysis of P4HA2 antibody (C-term) (Cat.# AP7393b) in A2058 cell line lysates (35ug/lane). P4HA2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with P4HA2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
P4HA2 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | P4HA2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P4HA2; Prolyl 4-hydroxylase subunit alpha-2; Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | P4HA2 Prolyl 4-hydroxylase subunit alpha-2 Procollagen-proline 2-oxoglutarate-4-dioxygenase subunit alpha-2 |
|---|---|
| Molecular Weight(MW) | 60902 Da |
| Function | Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins |
| Tissue Specificity | This P4HA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423~453 amino acids from the C-terminal region of human P4HA2. |
| Cellular Localization | Endoplasmic reticulum lumen. |
| Entrez Gene | 8974 |
|---|
Application
Western blot analysis of P4HA2 antibody (C-term) (Cat.# AP7393b) in A2058 cell line lysates (35ug/lane). P4HA2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with P4HA2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
P4HA2 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
