Application
A.Western blot analysis on (A)Raw264.7 , (B)MCF-7 and (C)HeLa cell lysate using anti-PABP2 RabMAb (Cat. #AJ1580a), dilution (A and B) 1:200,000 and (C) 1:1,000,000.Application
B.Immunohistochemical analysis of squamous cell cervical carcinoma using anti-PABP2 RabMAb (Cat. #AJ1580a).Application
C.Immunofliorescent staining of HeLa cells using anti-PABP2 RabMAb (Cat. #AJ1580a).| Product Name | PABP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PABPN1, PAB2, PABP2, Polyadenylate-binding protein 2, Polyadenylate-binding nuclear protein 1;Nuclear poly(A)-binding protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP3000Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAB2 PABP2 |
|---|---|
| Alternative Names | PABPN1 PAB2 PABP2 Polyadenylate-binding protein 2 Polyadenylate-binding nuclear protein 1 Nuclear poly(A)-binding protein 1 |
| Molecular Weight(MW) | 32749 Da |
| Function | Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation (By similarity) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles (By similarity) |
| Entrez Gene | 8106 |
|---|
Application
A.Western blot analysis on (A)Raw264.7 , (B)MCF-7 and (C)HeLa cell lysate using anti-PABP2 RabMAb (Cat. #AJ1580a), dilution (A and B) 1:200,000 and (C) 1:1,000,000.
Application
B.Immunohistochemical analysis of squamous cell cervical carcinoma using anti-PABP2 RabMAb (Cat. #AJ1580a).
Application
C.Immunofliorescent staining of HeLa cells using anti-PABP2 RabMAb (Cat. #AJ1580a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
