Application
Western blot analysis of hPACSIN3-T347 in mouse NIH-3T3 cell line and mouse cerebellum tissue lysates (35ug/lane). PACSIN3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
PACSIN3 Antibody (T347) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PACSIN3 antibody detected the PACSIN3 protein (arrow).Application
Western blot analysis of PACSIN3 (arrow) using rabbit polyclonal PACSIN3 Antibody (T347) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN3 gene.| Product Name | PACSIN3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PACSIN3; Protein kinase C and casein kinase substrate in neurons protein 3; Endophilin I; SH3 domain-containing protein 6511 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PACSIN3 Protein kinase C and casein kinase substrate in neurons protein 3 Endophilin I SH3 domain-containing protein 6511 |
|---|---|
| Molecular Weight(MW) | 48487 Da |
| Function | May play a role in endocytosis (By similarity). |
| Tissue Specificity | Widely expressed, with highest levels in heart and skeletal muscle, intermediate levels in placenta, liver and pancreas, and very low levels in brain, lung and kidney |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Note=Detected at the inner aspect of the plasma membrane in myotubes (By similarity) |
| Entrez Gene | 29763 |
|---|
Application
Western blot analysis of hPACSIN3-T347 in mouse NIH-3T3 cell line and mouse cerebellum tissue lysates (35ug/lane). PACSIN3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
PACSIN3 Antibody (T347) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PACSIN3 antibody detected the PACSIN3 protein (arrow).
Application
Western blot analysis of PACSIN3 (arrow) using rabbit polyclonal PACSIN3 Antibody (T347) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN3 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
