Application
A.Western blot analysis on (A) K562, (B) Jurkat and (C) HepG2 cell lysates using anti-PRKRA RabMAb (Cat. #AJ1581a), dilution 1:1000.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- PRKRA RabMAb (Cat. #AJ1581a).| Product Name | PACT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKRA, PACT, RAX, Interferon-inducible double stranded RNA-dependent protein kinase activator A, Protein kinase, interferon-inducible double stranded RNA-dependent activator;Protein activator of the interferon-induced protein kinase;PKR-associated pro |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3224 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PACT RAX |
|---|---|
| Alternative Names | PRKRA PACT RAX Interferon-inducible double stranded RNA-dependent protein kinase activator A Protein kinase interferon-inducible double stranded RNA-dependent activator Protein activator of the interferon-induced protein kinase PKR-associated pro |
| Molecular Weight(MW) | 34404 Da |
| Function | Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 |
| Tissue Specificity | A synthetic peptide corresponding to residues on the C terminus of human PACT was used as an immunogen. |
| Cellular Localization | Cytoplasm, perinuclear region. |
| Entrez Gene | 8575 |
|---|
Application
A.Western blot analysis on (A) K562, (B) Jurkat and (C) HepG2 cell lysates using anti-PRKRA RabMAb (Cat. #AJ1581a), dilution 1:1000.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti- PRKRA RabMAb (Cat. #AJ1581a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
