Application
Dot blot analysis of anti-Phospho-PRKRA-pS246 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-PRKRA-pS246 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKRA; PACT; RAX; Interferon-inducible double stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double s |
| Modification | p-S246 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PACT RAX |
|---|---|
| Alternative Names | PRKRA PACT RAX Interferon-inducible double stranded RNA-dependent protein kinase activator A PKR-associated protein X PKR-associating protein X Protein activator of the interferon-induced protein kinase Protein kinase interferon-inducible double s |
| Molecular Weight(MW) | 34404 Da |
| Function | Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 |
| Tissue Specificity | This Phospho-PRKRA-pS246 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 231~261 amino acids surrounding S246 of human PRKRA. |
| Cellular Localization | Cytoplasm, perinuclear region. |
| Entrez Gene | 8575 |
|---|
Application
Dot blot analysis of anti-Phospho-PRKRA-pS246 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
