Application
PAF1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PAF1 antibody detected the PAF1 protein (arrow).Application
Western blot analysis of PAF1 (arrow) using rabbit polyclonal PAF1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.| Product Name | PAF1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAF1; PD2; RNA polymerase II-associated factor 1 homolog; Pancreatic differentiation protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PD2 |
|---|---|
| Alternative Names | PAF1 PD2 RNA polymerase II-associated factor 1 homolog Pancreatic differentiation protein 2 |
| Molecular Weight(MW) | 59976 Da |
| Function | Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro |
| Tissue Specificity | This PAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human PAF1. |
| Cellular Localization | Nucleus. Note=Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin |
| Entrez Gene | 54623 |
|---|---|
| Nucleotide Accession | NP_061961.2 |
Application
PAF1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PAF1 antibody detected the PAF1 protein (arrow).
Application
Western blot analysis of PAF1 (arrow) using rabbit polyclonal PAF1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
