Application
Western blot analysis of hPAD4-M1 (RB07586) in K562, Hela, 293 cell line lysates (35ug/lane).PAD4 (arrow) was detected using the purified Pab.Application
Western blot analysis of PAD4 (arrow) using rabbit polyclonal PAD4 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PADI4 gene (Lane 2) (Origene Technologies).Application
Confocal immunofluorescent analysis of PAD4 Antibody (N-term)(Cat#AP2546a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | PAD4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PADI4; PADI5; PDI5; Protein-arginine deiminase type-4; HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PADI5 PDI5 |
|---|---|
| Alternative Names | PADI4 PADI5 PDI5 Protein-arginine deiminase type-4 HL-60 PAD Peptidylarginine deiminase IV Protein-arginine deiminase type IV |
| Molecular Weight(MW) | 74079 Da |
| Function | Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 |
| Tissue Specificity | Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils |
| Entrez Gene | 23569 |
|---|
Application
Western blot analysis of hPAD4-M1 (RB07586) in K562, Hela, 293 cell line lysates (35ug/lane).PAD4 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PAD4 (arrow) using rabbit polyclonal PAD4 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PADI4 gene (Lane 2) (Origene Technologies).
Application
Confocal immunofluorescent analysis of PAD4 Antibody (N-term)(Cat#AP2546a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
