Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PAK2 (pS20) RabMAb (Cat. #AJ1584b), 1:20,000 dilution. Cells were either (A) untreated (B) treated with calyculin A| Product Name | PAK2 Antibody Phospho (pS20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK2, PAK-2p34, C-t-PAK2 |
| Modification | p-S20 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR658(2) |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000~50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PAK2 PAK-2p34 C-t-PAK2 |
|---|---|
| Molecular Weight(MW) | 58043 Da |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues Full-length PAK2 stimulates cell survival and cell growth Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation |
| Tissue Specificity | Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen |
| Cellular Localization | Serine/threonine-protein kinase PAK 2: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane |
| Entrez Gene | 5062 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-PAK2 (pS20) RabMAb (Cat. #AJ1584b), 1:20,000 dilution. Cells were either (A) untreated (B) treated with calyculin A| Application Notes | WB:1:10000~50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
