Application
Western blot analysis of PAK3 (arrow) using PAK3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies).Application
PAK3 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PAK3 antibody detected the PAK3 protein (arrow).Application
Confocal immunofluorescent analysis of PAK3 Antibody (C-term)(Cat#AP7928d) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | PAK3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,IF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OPHN3 |
|---|---|
| Alternative Names | PAK3 OPHN3 Serine/threonine-protein kinase PAK 3 Beta-PAK Oligophrenin-3 p21-activated kinase 3 |
| Molecular Weight(MW) | 62310 Da |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments |
| Tissue Specificity | Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 5063 |
|---|
Application
Western blot analysis of PAK3 (arrow) using PAK3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies).
Application
PAK3 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PAK3 antibody detected the PAK3 protein (arrow).
Application
Confocal immunofluorescent analysis of PAK3 Antibody (C-term)(Cat#AP7928d) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
