Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PAPSS1 Antibody (N-term) (Cat. # AP8090a) to detect recombinant PAPSS1 (arrow). Ab dilution 1:50, using 2% skim milk for blocking. Data courtesy of L. Gebel, J.W. Mueller, P. Bayer, UniversitApplication
PAPSS1 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the PAPSS1 antibody detected the PAPSS1 protein (arrow).| Product Name | PAPSS1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAPSS1; ATPSK1; PAPSS; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; Sulfurylase kinase 1; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATPSK1 PAPSS |
|---|---|
| Alternative Names | PAPSS1 ATPSK1 PAPSS Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Sulfurylase kinase 1 Sulfate adenylyltransferase ATP-sulfurylase Sulfate adenylate transferase Adenylyl-sulfate kinase 3'-phosphoadenosine-5'-phosphosulfate synthase |
| Molecular Weight(MW) | 70833 Da |
| Function | Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells |
| Tissue Specificity | Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver Also expressed in high endothelial venules (HEV) cells and in cartilage |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PAPSS1 Antibody (N-term) (Cat. # AP8090a) to detect recombinant PAPSS1 (arrow). Ab dilution 1:50, using 2% skim milk for blocking. Data courtesy of L. Gebel, J.W. Mueller, P. Bayer, Universit
Application
PAPSS1 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the PAPSS1 antibody detected the PAPSS1 protein (arrow).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
