Application
Western blot analysis of PAPD1 Antibody (N-term) in Hela cell line lysates (35ug/lane). PAPD1 (arrow) was detected using the purified Pab.Application
PAPD1 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PAPD1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MTPAP; PAPD1; Poly(A) RNA polymerase, mitochondrial; PAP-associated domain-containing protein 1; Polynucleotide adenylyltransferase; Terminal uridylyltransferase 1; mtPAP |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100-500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAPD1 |
|---|---|
| Alternative Names | MTPAP PAPD1 Poly(A) RNA polymerase mitochondrial PAP-associated domain-containing protein 1 Polynucleotide adenylyltransferase Terminal uridylyltransferase 1 mtPAP |
| Molecular Weight(MW) | 66172 Da |
| Function | Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA |
| Tissue Specificity | Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle |
| Cellular Localization | Cytoplasm. Mitochondrion. |
| Entrez Gene | 55149 |
|---|
Application
Western blot analysis of PAPD1 Antibody (N-term) in Hela cell line lysates (35ug/lane). PAPD1 (arrow) was detected using the purified Pab.
Application
PAPD1 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100-500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
