Application
Western blot analysis of Parg (arrow) using rabbit polyclonal Parg Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Parg gene (Lane 2) (Origene Technologies).Application
Western blot analysis of Parg Antibody (C-term) in 293 cell line lysates (35ug/lane). Parg (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Parg antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Parg Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARG; Poly(ADP-ribose) glycohydrolase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PARG Poly(ADP-ribose) glycohydrolase |
|---|---|
| Molecular Weight(MW) | 111110 Da |
| Function | Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG acts both as an endo- and exoglycosidase, releasing PAR of different length as well as ADP-ribose monomers Required for retinoid acid-dependent gene transactivation, probably by dePARsylating histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Isoform 1: Nucleus. Note=Co-localizes with PCNA at replication foci. Relocalizes to the cytoplasm in response to DNA damage Isoform 3: Cytoplasm. Isoform 5: Mitochondrion matrix. |
| Entrez Gene | 8505 |
|---|
Application
Western blot analysis of Parg (arrow) using rabbit polyclonal Parg Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Parg gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of Parg Antibody (C-term) in 293 cell line lysates (35ug/lane). Parg (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Parg antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
