Application
Fresh frozen tissue samples from the human temporal lobe (Htl) is subjected to different extraction buffers(Htl/Low Salt, Htl/high salt, Htl/1%triton, Htl/RIPA, Htl/1%SDS, HEK293T-pcDNA3, HEK293T-hLRRK2). PARK8 (LRRK2) Antibody (E519) has sufficient specificity to detect endogenous human LRRK2.Application
PARK8 (LRRK2) Antibody (E519) detect over-expressed human LRRK2 protein.| Product Name | PARK8 (LRRK2) Antibody (E519) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LRRK2; PARK8; Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PARK8 |
|---|---|
| Alternative Names | LRRK2 PARK8 Leucine-rich repeat serine/threonine-protein kinase 2 Dardarin |
| Molecular Weight(MW) | 286103 Da |
| Function | May play a role in the phosphorylation of proteins central to Parkinson disease. Phosphorylates PRDX3. May also have GTPase activity. Positively regulates autophagy through a calcium- dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes |
| Tissue Specificity | Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen Expression is particularly high in brain dopaminoceptive areas |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Mitochondrion. Note=Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane |
Application
Fresh frozen tissue samples from the human temporal lobe (Htl) is subjected to different extraction buffers(Htl/Low Salt, Htl/high salt, Htl/1%triton, Htl/RIPA, Htl/1%SDS, HEK293T-pcDNA3, HEK293T-hLRRK2). PARK8 (LRRK2) Antibody (E519) has sufficient specificity to detect endogenous human LRRK2.
Application
PARK8 (LRRK2) Antibody (E519) detect over-expressed human LRRK2 protein.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
