Application
Western blot analysis of PARK8 (LRRK2) (arrow) using rabbit polyclonal PARK8 (LRRK2)Antibody (L899)(Cat# AP7099g). (A) 293 cell lysate (2 ug) transiently transfected with the PARK8 gene (Origene Technologies). (B) Mouse brain lysates (35ug/lane). PARK8 (arrow) was detected using the purified polyclonal antibody.Application
PARK8 (LRRK2)Antibody (L899)(Cat# AP7099g) detect over-expressed human LRRK2 protein.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PARK8 (LRRK2) Antibody (L899) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LRRK2; PARK8; Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PARK8 |
|---|---|
| Alternative Names | LRRK2 PARK8 Leucine-rich repeat serine/threonine-protein kinase 2 Dardarin |
| Molecular Weight(MW) | 286103 Da |
| Function | May play a role in the phosphorylation of proteins central to Parkinson disease. Phosphorylates PRDX3. May also have GTPase activity. Positively regulates autophagy through a calcium- dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes |
| Tissue Specificity | Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen Expression is particularly high in brain dopaminoceptive areas |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Mitochondrion. Note=Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane |
Application
Western blot analysis of PARK8 (LRRK2) (arrow) using rabbit polyclonal PARK8 (LRRK2)Antibody (L899)(Cat# AP7099g). (A) 293 cell lysate (2 ug) transiently transfected with the PARK8 gene (Origene Technologies). (B) Mouse brain lysates (35ug/lane). PARK8 (arrow) was detected using the purified polyclonal antibody.
Application
PARK8 (LRRK2)Antibody (L899)(Cat# AP7099g) detect over-expressed human LRRK2 protein.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
