Application
Western blot analysis of PARP16 Antibody (C-term) in HepG2,MDA-MB435 cell line lysates (35ug/lane).PARP16 (arrow) was detected using the purified Pab.Application
PARP16 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PARP16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PARP16 Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PARP16 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARP16; C15orf30; Poly [ADP-ribose] polymerase 16; ADP-ribosyltransferase diphtheria toxin-like 15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C15orf30 |
|---|---|
| Alternative Names | PARP16 C15orf30 Poly [ADP-ribose] polymerase 16 ADP-ribosyltransferase diphtheria toxin-like 15 |
| Molecular Weight(MW) | 36383 Da |
| Function | Mono-ADP-ribosyltransferase targeting the karyopherin KPNB1. Plays a role in unfolded protein response (UPR), by ADP- ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors |
| Tissue Specificity | This PARP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-250 amino acids from the C-terminal region of human PARP16. |
| Cellular Localization | Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein |
| Entrez Gene | 54956 |
|---|
Application
Western blot analysis of PARP16 Antibody (C-term) in HepG2,MDA-MB435 cell line lysates (35ug/lane).PARP16 (arrow) was detected using the purified Pab.
Application
PARP16 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PARP16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PARP16 Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
