Application
PARVB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PARVB antibody detected the PARVB protein (arrow).Application
PARVB Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PARVB antibody detected the PARVB protein (arrow).Application
Western blot analysis of PARVB (arrow) using rabbit polyclonal PARVB Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARVB gene.Application
PARVB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PARVB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PARVB Antibody (N-term)(Cat#AP11774a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
PARVB Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PARVB Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARVB; Beta-parvin; Affixin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PARVB Beta-parvin Affixin |
|---|---|
| Molecular Weight(MW) | 41714 Da |
| Function | Adapter protein that plays a role in integrin signaling via ILK and in activation of the GTPases CDC42 and RAC1 by guanine exchange factors, such as ARHGEF6. Is involved in the reorganization of the actin cytoskeleton and formation of lamellipodia. Plays a role in cell adhesion, cell spreading, establishment or maintenance of cell polarity, and cell migration |
| Tissue Specificity | Expressed predominantly in heart and skeletal muscle |
| Cellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cytoskeleton. Cell projection, lamellipodium Cytoplasm, myofibril, sarcomere. Cytoplasm, myofibril, sarcomere, Z line. Note=Constituent of focal adhesions. Detected at the tips of the leading edge of cells. Colocalizes with F-actin at the tips of lamellipodia |
| Entrez Gene | 29780 |
|---|---|
| Nucleotide Accession | NP_037459.2 |
Application
PARVB Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PARVB antibody detected the PARVB protein (arrow).
Application
PARVB Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PARVB antibody detected the PARVB protein (arrow).
Application
Western blot analysis of PARVB (arrow) using rabbit polyclonal PARVB Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PARVB gene.
Application
PARVB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PARVB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PARVB Antibody (N-term)(Cat#AP11774a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
PARVB Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
