Application
Western blot analysis of PATL1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). PATL1 (arrow) was detected using the purified Pab.Application
PATL1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PATL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PATL1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PATL1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PATL1; Protein PAT1 homolog 1; PAT1-like protein 1; Protein PAT1 homolog b |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PATL1 Protein PAT1 homolog 1 PAT1-like protein 1 Protein PAT1 homolog b |
|---|---|
| Molecular Weight(MW) | 86850 Da |
| Function | RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm, P-body. Nucleus. Nucleus, PML body. Nucleus speckle. Note=Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner Enriched in splicing speckles. Localization to nuclear foci and speckles requires active transcription. Excluded from the nucleolus |
| Entrez Gene | 219988 |
|---|
Application
Western blot analysis of PATL1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). PATL1 (arrow) was detected using the purified Pab.
Application
PATL1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PATL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PATL1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
