Application
Western blot analysis of PAX3 Antibody (N-term) in K562 cell line lysates (35ug/lane). PAX3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PAX3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAX3; HUP2; Paired box protein Pax-3; HuP2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HUP2 |
|---|---|
| Alternative Names | PAX3 HUP2 Paired box protein Pax-3 HuP2 |
| Molecular Weight(MW) | 52968 Da |
| Function | Probable transcription factor associated with development of alveolar rhabdomyosarcoma |
| Tissue Specificity | This PAX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105~134 amino acids from the N-terminal region of human PAX3. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5077 |
|---|
Application
Western blot analysis of PAX3 Antibody (N-term) in K562 cell line lysates (35ug/lane). PAX3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
