Application
Western blot analysis of PXN (arrow) using rabbit polyclonal PXN Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PXN gene (Lane 2) (Origene Technologies).Application
Western blot analysis of PXN Antibody (C-term) in Hela cell line lysates (35ug/lane). PXN (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PXN antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Paxillin (PXN) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PXN; Paxillin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PXN Paxillin |
|---|---|
| Molecular Weight(MW) | 64505 Da |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) |
| Tissue Specificity | This PXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559~589 amino acids from the C-terminal region of human PXN. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex (By similarity) Note=Colocalizes with integrins at the cell periphery (By similarity) |
| Entrez Gene | 5829 |
|---|
Application
Western blot analysis of PXN (arrow) using rabbit polyclonal PXN Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PXN gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of PXN Antibody (C-term) in Hela cell line lysates (35ug/lane). PXN (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PXN antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
