Application
A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-Paxillin (pY31)RabMAb (Cat. #AJ1592c); dilution 1:10,000. Cells were (1) untreated, and (2) treated with PDGF.Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-Phospho-Paxillin (pY31) RabMAb (Cat. #AJ1592c).Application
C. Immunofluorescent staining of HeLa cells using anti-Phospho-Paxillin (pY31) RabMAb (Cat. #AJ1592c).| Product Name | Paxillin Antibody Phospho (pY31) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PXN, Paxillin |
| Modification | p-Y31 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E228 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PXN Paxillin |
|---|---|
| Molecular Weight(MW) | 64505 Da |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex (By similarity) Note=Colocalizes with integrins at the cell periphery (By similarity) |
| Entrez Gene | 5829 |
|---|
Application
A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-Paxillin (pY31)RabMAb (Cat. #AJ1592c); dilution 1:10,000. Cells were (1) untreated, and (2) treated with PDGF.
Application
B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-Phospho-Paxillin (pY31) RabMAb (Cat. #AJ1592c).
Application
C. Immunofluorescent staining of HeLa cells using anti-Phospho-Paxillin (pY31) RabMAb (Cat. #AJ1592c).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
