Application
Western blot analysis of anti-PBK Antibody (N-term N6) in T47D cell line lysates (35ug/lane). PBK(arrow) was detected using the purified Pab (1:210 dilution).Application
Western blot analysis of PBK/TOPK(arrow) using rabbit polyclonal PBK/TOPK Antibody (Mouse N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK/TOPK gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PBK Antibody (N-term N6) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; T-LAK cell-originated protein kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~250 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TOPK |
|---|---|
| Alternative Names | PBK TOPK Lymphokine-activated killer T-cell-originated protein kinase Cancer/testis antigen 84 MAPKK-like protein kinase Nori-3 PDZ-binding kinase Spermatogenesis-related protein kinase T-LAK cell-originated protein kinase |
| Molecular Weight(MW) | 36085 Da |
| Function | Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage |
| Tissue Specificity | Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules |
| Entrez Gene | 55872 |
|---|
Application
Western blot analysis of anti-PBK Antibody (N-term N6) in T47D cell line lysates (35ug/lane). PBK(arrow) was detected using the purified Pab (1:210 dilution).
Application
Western blot analysis of PBK/TOPK(arrow) using rabbit polyclonal PBK/TOPK Antibody (Mouse N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK/TOPK gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~250 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
