Application
Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PCAT1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PCAT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LPCAT1; AYTL2; PFAAP3; Lysophosphatidylcholine acyltransferase 1; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acyltransferase-like 2; Phospho |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AYTL2 PFAAP3 |
|---|---|
| Alternative Names | LPCAT1 AYTL2 PFAAP3 Lysophosphatidylcholine acyltransferase 1 1-acylglycerophosphocholine O-acyltransferase 1-alkylglycerophosphocholine O-acetyltransferase Acetyl-CoA:lyso-platelet-activating factor acetyltransferase Acyltransferase-like 2 Phospho |
| Molecular Weight(MW) | 59151 Da |
| Function | Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology |
| Tissue Specificity | This PCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505~534 amino acids from the C-terminal region of human PCAT1. |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein (By similarity). Golgi apparatus membrane; Single-pass type II membrane protein (By similarity) |
| Entrez Gene | 79888 |
|---|
Application
Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PCAT1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
