Application
PCDH20 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCDH20 antibody detected the PCDH20 protein (arrow).Application
PCDH20 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PCDH20 Antibody (Center)(Cat#AP11655c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PCDH20 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCDH20; PCDH13; Protocadherin-20; Protocadherin-13 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PCDH13 |
|---|---|
| Alternative Names | PCDH20 PCDH13 Protocadherin-20 Protocadherin-13 |
| Molecular Weight(MW) | 101927 Da |
| Function | Potential calcium-dependent cell-adhesion protein. |
| Tissue Specificity | This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-480 amino acids from the Central region of human PCDH20. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 64881 |
|---|---|
| Nucleotide Accession | NP_073754.2 |
Application
PCDH20 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCDH20 antibody detected the PCDH20 protein (arrow).
Application
PCDH20 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PCDH20 Antibody (Center)(Cat#AP11655c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
