Application
Western blot analysis on (A) HeLa, (B) HepG2 and (C) 293T cell lysates using anti-PCNA RabMAb (Cat. #AJ1594b).Application
Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-PCNA RabMAb (Cat. #AJ1594b).Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA RabMAb (Cat. #AJ1594b).| Product Name | PCNA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCNA, Proliferating cell nuclear antigen, Cyclin |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3822 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PCNA Proliferating cell nuclear antigen Cyclin |
|---|---|
| Molecular Weight(MW) | 28769 Da |
| Function | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C terminus in human PCNA was used as an immunogen. |
| Cellular Localization | Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents |
| Entrez Gene | 5111 |
|---|
Application
Western blot analysis on (A) HeLa, (B) HepG2 and (C) 293T cell lysates using anti-PCNA RabMAb (Cat. #AJ1594b).
Application
Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-PCNA RabMAb (Cat. #AJ1594b).
Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA RabMAb (Cat. #AJ1594b).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
