Application
Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.Application
Confocal immunofluorescent analysis of PCK2 Antibody (N-term)(Cat#AP8094a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | PCK2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCK2; PEPCK2; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; Phosphoenolpyruvate carboxylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PEPCK2 |
|---|---|
| Alternative Names | PCK2 PEPCK2 Phosphoenolpyruvate carboxykinase [GTP] mitochondrial Phosphoenolpyruvate carboxylase |
| Molecular Weight(MW) | 70730 Da |
| Function | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity) |
| Tissue Specificity | This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~62 amino acids from the N-terminal region of human PCK2. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 5106 |
|---|
Application
Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.
Application
Confocal immunofluorescent analysis of PCK2 Antibody (N-term)(Cat#AP8094a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
