Application
Western blot analysis of PCM-1 antibody (C-eterm) (Cat.# AP7481b) in K562 cell line lysates (35ug/lane). PCM1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using PCM-1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PCM-1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCM1; Pericentriolar material 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PCM1 Pericentriolar material 1 protein |
|---|---|
| Molecular Weight(MW) | 228533 Da |
| Function | Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia |
| Tissue Specificity | Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Cytoplasmic granule. Note=Localizes to cytoplasmic granules which are enriched around the centrosome This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase |
| Entrez Gene | 5108 |
|---|
Application
Western blot analysis of PCM-1 antibody (C-eterm) (Cat.# AP7481b) in K562 cell line lysates (35ug/lane). PCM1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using PCM-1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
