Application
PCNA Antibody (C-term) (Cat. #AX10001) western blot analysis in MDA-MB231, Jurkat, K562, MDA-MB453, 293 cell line lysates (20ug/lane). This demonstrates the PCNA antibody detected the PCNA protein (arrow).Application
PCNA Antibody (C-term) (Cat. #AX10001) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PCNA Antibody (C-term) (Cat. #AX10001)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PCNA Antibody (C-term) (Cat. #AX10001)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lymphnode(mesenteric) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PCNA Antibody (C-term)(Cat. #AX10001) immunohistochemistry analysis in formalin fixed and paraffin embedded rat lymphnode(mandibular) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PCNA Antibody (C-term)(Cat. #AX10001)flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PCNA Antibody (C-term) (Cat. #AX10001)with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | PCNA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCNA; Proliferating cell nuclear antigen; Cyclin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Clone Number | RB17486 |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PCNA Proliferating cell nuclear antigen Cyclin |
|---|---|
| Molecular Weight(MW) | 28769 Da |
| Function | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion |
| Tissue Specificity | This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~261 amino acids from the C-terminal region of human PCNA . |
| Cellular Localization | Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents |
| Entrez Gene | 5111 |
|---|
Application
PCNA Antibody (C-term) (Cat. #AX10001) western blot analysis in MDA-MB231, Jurkat, K562, MDA-MB453, 293 cell line lysates (20ug/lane). This demonstrates the PCNA antibody detected the PCNA protein (arrow).
Application
PCNA Antibody (C-term) (Cat. #AX10001) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PCNA Antibody (C-term) (Cat. #AX10001)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PCNA Antibody (C-term) (Cat. #AX10001)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lymphnode(mesenteric) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PCNA Antibody (C-term)(Cat. #AX10001) immunohistochemistry analysis in formalin fixed and paraffin embedded rat lymphnode(mandibular) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PCNA Antibody (C-term)(Cat. #AX10001)flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PCNA Antibody (C-term) (Cat. #AX10001)with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
