Application
The anti-PCPTP1 Pab is used in Western blot to detect PCPTP1 in placenta tissue lysate.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with hPCPTP1-Y249, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PCPTP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPRR; ECPTP; PTPRQ; Receptor-type tyrosine-protein phosphatase R; Ch-1PTPase; NC-PTPCOM1; Protein-tyrosine phosphatase PCPTP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ECPTP PTPRQ |
|---|---|
| Alternative Names | PTPRR ECPTP PTPRQ Receptor-type tyrosine-protein phosphatase R Ch-1PTPase NC-PTPCOM1 Protein-tyrosine phosphatase PCPTP1 |
| Molecular Weight(MW) | 73834 Da |
| Function | Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus (By similarity) |
| Tissue Specificity | Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus |
| Cellular Localization | Isoform Alpha: Cell membrane; Single-pass type I membrane protein Isoform Gamma: Cytoplasm, perinuclear region. Note=Locates to the perinuclear areas within the cytoplasm |
| Entrez Gene | 5801 |
|---|
Application
The anti-PCPTP1 Pab is used in Western blot to detect PCPTP1 in placenta tissue lysate.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with hPCPTP1-Y249, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
