Application
A. Western blot analysis on (A) Jurkat (B) HL-60 + RA and (C) HeLa cell lysate using anti-PDCD4 RabMAb (Cat. #AJ1595b), dilution 1:100,000.Application
B. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PDCD4 RabMAb (Cat. #AJ1595b).| Product Name | PDCD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCD4, H731, Programmed cell death protein 4, Nuclear antigen H731-like;Neoplastic transformation inhibitor protein;Protein 197/15a |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3431 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H731 |
|---|---|
| Alternative Names | PDCD4 H731 Programmed cell death protein 4 Nuclear antigen H731-like Neoplastic transformation inhibitor protein Protein 197/15a |
| Molecular Weight(MW) | 51735 Da |
| Function | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity) |
| Tissue Specificity | Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum |
| Entrez Gene | 27250 |
|---|
Application
A. Western blot analysis on (A) Jurkat (B) HL-60 + RA and (C) HeLa cell lysate using anti-PDCD4 RabMAb (Cat. #AJ1595b), dilution 1:100,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PDCD4 RabMAb (Cat. #AJ1595b).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
