Application
PDCD4 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PDCD4 antibody detected the PDCD4 protein (arrow).Application
PDCD4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PDCD4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PDCD4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCD4; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H731 |
|---|---|
| Alternative Names | PDCD4 H731 Programmed cell death protein 4 Neoplastic transformation inhibitor protein Nuclear antigen H731-like Protein 197/15a |
| Molecular Weight(MW) | 51735 Da |
| Function | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity) |
| Tissue Specificity | Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum |
| Entrez Gene | 27250 |
|---|---|
| Nucleotide Accession | NP_055271.2 NP_663314.1 |
Application
PDCD4 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PDCD4 antibody detected the PDCD4 protein (arrow).
Application
PDCD4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PDCD4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
