Application
Western blot analysis of PDHB Antibody (Center) in HepG2 cell line lysates (35ug/lane). PDHB (arrow) was detected using the purified Pab.Application
PDHB Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PDHB Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PDHB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDHB; PHE1B; Pyruvate dehydrogenase E1 component subunit beta, mitochondrial |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PHE1B |
|---|---|
| Alternative Names | PDHB PHE1B Pyruvate dehydrogenase E1 component subunit beta mitochondrial |
| Molecular Weight(MW) | 39233 Da |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle |
| Tissue Specificity | This PDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human PDHB. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5162 |
|---|
Application
Western blot analysis of PDHB Antibody (Center) in HepG2 cell line lysates (35ug/lane). PDHB (arrow) was detected using the purified Pab.
Application
PDHB Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PDHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PDHB Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
