Application
Western blot analysis of PDIA3 Antibody (Center) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)Application
Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using PDIA3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PDIA3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDIA3; ERP57; ERP60; GRP58; Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERP57 ERP60 GRP58 |
|---|---|
| Alternative Names | PDIA3 ERP57 ERP60 GRP58 Protein disulfide-isomerase A3 58 kDa glucose-regulated protein 58 kDa microsomal protein Disulfide isomerase ER-60 Endoplasmic reticulum resident protein 57 Endoplasmic reticulum resident protein 60 |
| Molecular Weight(MW) | 56782 Da |
| Tissue Specificity | Detected in the flagellum and head region of spermatozoa (at protein level) |
| Cellular Localization | Endoplasmic reticulum lumen (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 2923 |
|---|
Application
Western blot analysis of PDIA3 Antibody (Center) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using PDIA3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
