Application
A. Western blot analysis on A) human heart B) mouse heart C) rat heart tissue lysate using anti-PDK2 RabMAb (Cat. #AJ1598b), dilution 1:2000Application
B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-PDK2 RabMAb (Cat. #AJ1598b)| Product Name | PDK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK2, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1948Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:10000~100000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDHK2 |
|---|---|
| Alternative Names | PDK2 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 mitochondrial Pyruvate dehydrogenase kinase isoform 2 |
| Molecular Weight(MW) | 46154 Da |
| Function | Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis |
| Tissue Specificity | Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5164 |
|---|
Application
A. Western blot analysis on A) human heart B) mouse heart C) rat heart tissue lysate using anti-PDK2 RabMAb (Cat. #AJ1598b), dilution 1:2000
Application
B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-PDK2 RabMAb (Cat. #AJ1598b)| Application Notes | WB:10000~100000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
