Application
Western blot analysis of PDK2 Antibody (C-term) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab.Application
Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).Application
PDK2 Antibody (C392) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).| Product Name | PDK2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; Pyruvate dehydrogenase kinase isoform 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDHK2 |
|---|---|
| Alternative Names | PDK2 PDHK2 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 mitochondrial Pyruvate dehydrogenase kinase isoform 2 |
| Molecular Weight(MW) | 46154 Da |
| Function | Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis |
| Tissue Specificity | Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5164 |
|---|
Application
Western blot analysis of PDK2 Antibody (C-term) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).
Application
PDK2 Antibody (C392) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
