Application
Western blot analysis of PEA-15 Antibody (C-term) in T47D cell line lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab.Application
Western blot analysis of PEA-15 Antibody (C-term) in mouse lung tissue lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded mouse heart tissue reacted with PEA-15 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PEA-15 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PEA-15 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PEA15; Astrocytic phosphoprotein PEA-15; 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PEA15 Astrocytic phosphoprotein PEA-15 15 kDa phosphoprotein enriched in astrocytes Phosphoprotein enriched in diabetes |
|---|---|
| Molecular Weight(MW) | 15040 Da |
| Function | Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity) Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface |
| Tissue Specificity | Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose- producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls |
| Cellular Localization | Cytoplasm. Note=Associated with microtubules |
| Entrez Gene | 8682 |
|---|
Application
Western blot analysis of PEA-15 Antibody (C-term) in T47D cell line lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PEA-15 Antibody (C-term) in mouse lung tissue lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded mouse heart tissue reacted with PEA-15 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PEA-15 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
