Application
PELI3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PELI3 antibody detected the PELI3 protein (arrow).Application
PELI3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PELI3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PELI3; E3 ubiquitin-protein ligase pellino homolog 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PELI3 E3 ubiquitin-protein ligase pellino homolog 3 |
|---|---|
| Molecular Weight(MW) | 50755 Da |
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation |
| Tissue Specificity | Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung |
| Entrez Gene | 246330 |
|---|---|
| Nucleotide Accession | NP_001091980.1 NP_659502.2 |
Application
PELI3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PELI3 antibody detected the PELI3 protein (arrow).
Application
PELI3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
