Application
PELP1 Antibody (Center R759) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PELP1 antibody detected the PELP1 protein (arrow).Application
PELP1 Antibody (Center R759) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PELP1 Antibody (Center R759) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PELP1 Antibody (Center R759) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PELP1; HMX3; MNAR; Proline-, glutamic acid- and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMX3 MNAR |
|---|---|
| Alternative Names | PELP1 HMX3 MNAR Proline- glutamic acid- and leucine-rich protein 1 Modulator of non-genomic activity of estrogen receptor Transcription factor HMX3 |
| Molecular Weight(MW) | 119700 Da |
| Function | Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes |
| Tissue Specificity | Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed |
| Cellular Localization | Nucleus, nucleoplasm (By similarity). Nucleus. Cytoplasm. Note=Mainly found in the nucleoplasm, with low levels detected in the cytoplasm (By similarity). Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors |
Application
PELP1 Antibody (Center R759) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PELP1 antibody detected the PELP1 protein (arrow).
Application
PELP1 Antibody (Center R759) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PELP1 Antibody (Center R759) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
