Application
Western blot analysis of PERK Antibody (N-term Q163) in 293 cell line lysates (35ug/lane). PERK (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PERK Antibody (N-term Q163) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PEK PERK |
|---|---|
| Alternative Names | EIF2AK3 PEK PERK Eukaryotic translation initiation factor 2-alpha kinase 3 PRKR-like endoplasmic reticulum kinase Pancreatic eIF2-alpha kinase |
| Molecular Weight(MW) | 125216 Da |
| Function | Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (By similarity) |
| Tissue Specificity | Ubiquitous. A high level expression is seen in secretory tissues |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
| Entrez Gene | 9451 |
|---|
Application
Western blot analysis of PERK Antibody (N-term Q163) in 293 cell line lysates (35ug/lane). PERK (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
