Application
PFN1 Antibody western blot analysis in MDA-MB435,Jurkat,Hela cell line lysates (15ug/lane).This demonstrates the PFN1 antibody detected the PFN1 protein (arrow).Application
PFN1 Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PFN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PFN1; Profilin-1; Epididymis tissue protein Li 184a; Profilin I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PFN1 Profilin-1 Epididymis tissue protein Li 184a Profilin I |
|---|---|
| Molecular Weight(MW) | 15054 Da |
| Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR |
| Tissue Specificity | Expressed in epididymis (at protein level). |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 5216 |
|---|---|
| Nucleotide Accession | NP_005013.1 |
Application
PFN1 Antibody western blot analysis in MDA-MB435,Jurkat,Hela cell line lysates (15ug/lane).This demonstrates the PFN1 antibody detected the PFN1 protein (arrow).
Application
PFN1 Antibody flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
