Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with PFN1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-PFN1 Antibody (Center) in A2058 cell line lysates (35ug/lane). PFN1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PFN1 (arrow) using rabbit polyclonal PFN1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PFN1 gene.| Product Name | PFN1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PFN1; Profilin-1; Epididymis tissue protein Li 184a; Profilin I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PFN1 Profilin-1 Epididymis tissue protein Li 184a Profilin I |
|---|---|
| Molecular Weight(MW) | 15054 Da |
| Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR |
| Tissue Specificity | Expressed in epididymis (at protein level). |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 5216 |
|---|
Application
Formalin-fixed and paraffin-embedded human lymph tissue reacted with PFN1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-PFN1 Antibody (Center) in A2058 cell line lysates (35ug/lane). PFN1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PFN1 (arrow) using rabbit polyclonal PFN1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PFN1 gene.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
